Notch Signaling and Somite Formation

One of the key agents in determining where somites form is the Notch signaling pathway (see Aulehla and Pourquié 2008). When a small group of cells from a region constituting the posterior border at the presumptive somite boundary is transplanted into a region of presomitic mesoderm that would not ordinarily be part of the boundary area, a new boundary is created. The transplanted boundary cells instruct the cells anterior to them to epithelialize and separate. Nonboundary cells will not induce border formation when transplanted to a nonborder area. However, these cells can acquire boundary- forming ability if an activated Notch protein is electroporated into them (Figure 1; Sato et al. 2002). Morimoto and colleagues (2005) have been able to visualize the endogenous level of Notch activity in mouse embryos and have shown that it oscillates in a segmentally defined pattern. The somite boundaries were formed at the interface between the Notch- expressing and Notch-nonexpressing areas. The expression pattern of Hairy1—a segmentation gene regulated by Notch activity—at the anterior of the presomitic mesoderm at the end of each cycle of expression was exactly where the transplantation experiments showed Notch expression to be important (see textbook Figure 17.15).

Figure 1 Notch signaling and somite formation. (A) Transplantation of a prospective somite boundary region into a nonboundary region creates a new boundary and a new somite. The transplanted quail cells can be identified by staining for a quail-specific protein. (B) Transplantation of nonboundary cells into a nonboundary region does not create a new boundary or a new somite. (C) Transplantation of a nonboundary region that has had Notch activated will cause a new somite boundary to occur. (After Sato et al. 2002, photographs courtesy of Y. Takahashi.)

Literature Cited

Aulehla, A. and O. Pourquié. 2008. Oscillating signaling pathways during embryonic development. Curr. Opin. Cell Biol. 20: 632–737.
PubMed Link

Morimoto, M., Y. Takahashi, M. Endo and Y. Saga. 2005. The Mesp2 transcription factor establishes segmental borders by suppressing Notch activity. Nature 435: 354–359.
PubMed Link

Sato, Y., K. Yasuda and Y. Takahashi. 2002. Morphological boundary forms by a novel inductive event mediated by Lunatic-Fringe and Notch during somitic segmentation. Development 129: 3633–3644.